Fig. 10. Knockdown effects of F-actin-capping and -severing factors on contact inhibition of proliferation. The knockdown of these factors can rescue the contact inhibition of proliferation. (A) Immunofluorescence imaging of YAP/TAZ with anti-YAP/TAZ antibody (αYAP/TAZ). Cells transfected with inhibitory siRNA (si Capzb, siCfl1, siGsn, and siCO that is relative to GAPDH expression) and seeded to obtain sparse cells or a dense monolayer. Loss of Capzb, Cfl1, or Gsn, as F-actin-capping and -severing factors, lead to YAP/TAZ nuclear localization in dense monolayers. DAPI is a nuclear counterstain. Scale bar, 20 μm. (B) The proportion of cells displaying preferential nuclear YAP/TAZ localization (N, black); even distribution of YAP/TAZ between the nucleus and the cytoplasm (N/C, gray); or cytoplasmic YAP/TAZ (C, white) (Reprinted with permission, Cell Press, for citation see [53]).